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Ninety-three from 178 isolates (52%) together with Twenty distinct 16S rRNA gene series could be used on validly released varieties. Both the invert collection soak up assays presented bogus identifications for 3 lately described types and Twenty two inacucuracy were noted in the id benefits backward and forward invert collection bare assays. Recognition by invert line blot assays underestimates t
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01?p.p.m. The protein extraction was performed on 400 quiescent, germinating and GR24-treated wt and cured spores for each experiment, according to the procedure described by Bestel-Corre and colleagues (2002). The pellet was dried and resuspended in 800??l of solubilization buffer containing 9?M urea, 2% Triton X-100. Lipids and nucleic acids were removed by supercentrifuging at 25?krpm during 30
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S.Supplies and Approaches Cell strains and culturesChronic myeloid leukemia KS.Products and Methods Cell strains and culturesChronic myeloid leukemia K562 [43], acute myeloid leukemia Kasumi-1 [44], and acute promyelocytic leukemia HL60 [45] cell strains were cultured in RPMI1640 medium supplemented with ten FBS. Lung cancer LU99B [46], gastric most cancers AZ-521 [47], fibrosarcoma HT-1080
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