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Some (At1g59860), HSP70B (At1g16030) and also HSP90.1 (At5g52640). The project had been supported by Us all Country wide Science Base scholarships IOS0919745 and MCB0950242 in order to L.Z ., and also by All of us Country wide Technology Basis allow DBI0922650. Number S1. Term regarding miR169, miR393 and also SPL7 under temperature strain, and warmth induction of miR398 under low-sucrose and also
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01?p.p.m. The protein extraction was performed on 400 quiescent, germinating and GR24-treated wt and cured spores for each experiment, according to the procedure described by Bestel-Corre and colleagues (2002). The pellet was dried and resuspended in 800??l of solubilization buffer containing 9?M urea, 2% Triton X-100. Lipids and nucleic acids were removed by supercentrifuging at 25?krpm during 30
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