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Immunoprecipitations have been accomplished using a polyclonal anti-GFP antibody (abcam-290; Abcam, http://www.abcam.net) destined right away for you to proteins Any agarose drops (Invitrogen). Proteins extracts have been incubated using the earlier mentioned preparing pertaining to One particular.5?h. Most experiments had been carried out in 4��C. Immunoprecipitates ended up washed 4 #links# time

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