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After trypsinization and a couple flushes by simply PBS, cells were fixed in frosty 70% ethanol in ?20?��C instantaneously, then incubated within a propidium iodide (Private investigator) mixture (50?��g/ml PI, 0.1?mg/ml RNase A, 2.05% Triton X-100 throughout PBS) with 37?��C with regard to 30?min. Fixed cells had been analyzed by the FACsort movement cytometer (BD Biosciences). Information order

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