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After trypsinization as well as washes simply by PBS, cellular material have been set throughout ice cold 70% ethanol at ?20?��C right away, after which incubated in the propidium iodide (Private detective) blend (50?��g/ml PI, 2.1?mg/ml RNase Any, 2.05% Triton X-100 throughout PBS) in 37?��C with regard to 30?min. Preset tissue were analyzed through the FACsort circulation cytometer (BD Bioscienc

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