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After trypsinization and two wipes by PBS, tissue had been repaired within chilled 70% ethanol from ?20?��C right away, and after that incubated in the propidium iodide (Private investigator) combination (50?��g/ml Private eye, 2.1?mg/ml RNase Any, 3.05% Triton X-100 within PBS) from 37?��C pertaining to 30?min. Preset tissues have been assessed by the FACsort stream cytometer (BD Biosciences). In

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